abstract：:In cytomegalovirus-infected cells, the rate of protein synthesis was detected as two peaks. One occurred during the early phase of infection, 0 to 36 h postinfection, and the other occurred during the late phase, after the initiation of viral DNA synthesis. Double-isotopic-label difference analysis demonstrated that h...

journal_title：Journal of virology

authors： Stinski MF

更新日期：1977-09-01 00:00:00

abstract：:Phage XP-12, which has complete substitution of the cytosine residues in its DNA with 5-methylcytosine residues, was shown to inhibit incorporation of uracil into host DNA and RNA during the latent period. This apparent inhibition of host macromolecular synthesis was not accompanied by extensive degradation of the hos...

journal_title：Journal of virology

authors： Ehrlich M,Lin FH,Ehrlich K,Brown SL,Mayo JA

更新日期：1977-09-01 00:00:00

abstract：:The effect DNA repair might have on the integration of exogenous proviral DNA into host cell DNA was investigated by comparing the efficiency of proviral DNA integration in normal chicken embryonic fibroblasts and in chicken embryonic fibroblasts treated with UV or 4-nitroquinoline-1-oxide. The cells were treated with...

journal_title：Journal of virology

authors： Tsuruo T,Baluda MA

更新日期：1977-09-01 00:00:00

abstract：:The possible addition of extra sequences to simian virus 40 (SV40) DNA was analyzed by electron microscopy in two different cell systems, productively infected monkey cells and activated heterokaryons on monkey and transformed mouse 3T3 cells. We found that the closed circular DNA fraction, extracted from monkey cells...

journal_title：Journal of virology

authors： Yoshike K,Defendi V

更新日期：1977-08-01 00:00:00

abstract：:The major envelope glycoprotein of bovine leukemia virus was isolated by lectin-bound Sepharose and DEAE-cellulose column chromatography. This protein was shown to have a molecular weight of about 41,000 and to lack detectable immunological cross-reactivity with glycoproteins of other oncornaviruses. Sera obtained fro...

journal_title：Journal of virology

authors： Devare SG,Stephenson JR

更新日期：1977-08-01 00:00:00

abstract：:Serial passage of reovirus temperature-sensitive (ts) mutant C(447) produced by passage 9 (P9) a heavily defective population of virus from which the double-stranded RNA genomic segments L(1), L(3), and M(1) were largely missing. Viral cores obtained from this P9 population were heterogeneous with respect to buoyant d...

journal_title：Journal of virology

authors： Ahmed R,Graham AF

更新日期：1977-08-01 00:00:00

abstract：:Virus-specific RNA synthesized in monkey cells after infection by both wild-type simian virus 40 (SV40) and the early SV40 temperature-sensitive mutant tsA58 has been analyzed. The fraction of SV40-specific RNA increased throughout infection with either wild-type SV40 or with tsA58 in direct proportion to the accumula...

journal_title：Journal of virology

authors： Khoury G,May E

更新日期：1977-07-01 00:00:00

abstract：:Growing CV1 cells were infected with simian virus 40 (SV40), and the levels of DNA polymerases-alpha, -beta, and -gamma were analyzed in the cytoplasm, nuclear Triton wash, and nucleus. In the cytoplasmic fraction, the amount of alpha-, beta-, or gamma-polymerase remained unaltered after SV40 infection. The activity o...

journal_title：Journal of virology

authors： Méchali M,Girard M,de Recondo AM

更新日期：1977-07-01 00:00:00

abstract：:RNA-dependent RNA polymerases were found to be associated with two salmonid rhabdoviruses: infectious hematopoietic necrosis (IHN) virus and the virus of hemorrhagic septicemia (VHS). The protein composition of these rhabdoviruses closely resembles that of rabies virus rather than that of vesicular stomatitis virus (M...

journal_title：Journal of virology

authors： McAllister PE,Wagner RR

更新日期：1977-06-01 00:00:00

abstract：:The lymphoid cell line #1670 has been derived from the infiltrated spleen of a tumor-bearing marmoset monkey infected with Herpesvirus saimiri. The cells contain both types of H. saimiri DNA, unique light (L-) DNA (36% cytosine plus guanine) and repetitive heavy (H-) DNA (71% cytosine plus guanine), without producing ...

journal_title：Journal of virology

authors： Werner FJ,Bornkamm GW,Fleckenstein B

更新日期：1977-06-01 00:00:00

abstract：:Immune precipitation with monospecific antiserum was employed to study the intracellular synthesis of viral glycoproteins gp85 and gp37. Labeled gp85 and gp37 were detected from lysates of cells transformed with Rous sacroma virus, strain B77, after long-term labeling with radioactive glucosamine or phenylalanine. Imm...

journal_title：Journal of virology

authors： Moelling K,Hayami M

更新日期：1977-06-01 00:00:00

abstract：:T7+ phage are unable to plate on a strain of Shigella sonnei D2 371-48. Spontaneous phage mutants arise (ss--mutants) that are able to plate on this strain of Shigella. We have shown by complementation studies and genetic crosses that the ss--mutation maps in gene 10, the structural gene for the major protein of the c...

journal_title：Journal of virology

authors： Vetter D,Roberts L,Jackowski JB,Sadowski PD

更新日期：1977-06-01 00:00:00

abstract：:Isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. The envelope protein and nucleocapsid protein were isolated at isoelectric points of pI 7.8 and 10.3, respectively. The antigenic determinants ...

journal_title：Journal of virology

authors： Trent DW

更新日期：1977-06-01 00:00:00

abstract：:Mitochondrial DNA synthesis in adenovirus type 2-infected HeLa cells was measured at various times from 0 to 24 h postinfection. Although viral infection effectively turned off host chromosomal DNA synthesis, mitochondrial DNA synthesis was not inhibited. These findings indicate a dissociation between the regulation o...

journal_title：Journal of virology

authors： Fisher PB,Horwitz MS

更新日期：1977-05-01 00:00:00

abstract：:We established previously that the temperature-dependent host range mutant, td CE 3, of vesicular stomatitis virus (VSV) New Jersey possesses temperature-sensitive RNA transcriptase activity. In this paper, we describe dissociation and reconstitution experiments designed to determine which VSV polypeptide is affected ...

journal_title：Journal of virology

authors： Szilágyi JF,Pringle CR,Macpherson TM

更新日期：1977-05-01 00:00:00

abstract：:Nine pre-early polypeptides have been detected after infection with bacteriophage T5 and 10 pre-early polypeptides have been detected after infection with bacteriophage BF23. Only about one-half of the coding capacity of the redundant ends of the phage DNA, which code for pre-early proteins, is needed for these 9 to 1...

journal_title：Journal of virology

authors： McCorquodale DJ,Shaw AR,Shaw PK,Chinnadurai G

更新日期：1977-05-01 00:00:00

abstract：:5' S-isobutyl-adenosine (SIBA), a structural analogue of S-adenosylhomocysteine, reversibly blocks the multiplication of herpes simplex type 1 virus. In the presence of SIBA, viral protein synthesis is inhibited. After removing SIBA the synthesis of proteins starts rapidly again. The new polypeptides are mainly alpha ...

journal_title：Journal of virology

authors： Jacquemont B,Huppert J

更新日期：1977-04-01 00:00:00

abstract：:Antigens detected by the complement-fixation (CF) test were prepared from BHK-21 cells infected with Pichinde virus. The preparations contained two antigens demonstrable by immunodiffusion. The antigen present in abundance was heat stable, Pronase resistant, and had a molecular weight of 20,000 to 30,000 as estimated ...

journal_title：Journal of virology

authors： Buchmeier MJ,Gee SR,Rawls WE

更新日期：1977-04-01 00:00:00

abstract：:The level of RNase activity in human interferon preparations was examined. Although sequential purification of interferon resulted in nearly a 300-fold increase in specific activity, RNase-specific activity remained more or less constant. The implications of this finding for the analyses of the mode of action of inter...

journal_title：Journal of virology

authors： Winchurch RA,Karpetsky TP,Levy CC,Cantell K

更新日期：1977-03-01 00:00:00

abstract：:The cocultivation of a lung cell line from the Southeast Asian mouse Mus cervicolor with cells from heterologous species has resulted in the isolation of two new distinct type C viruses. Both viruses are endogenous to M. cervicolor and are present in multiple copies in the cellular DNA of these mice. One of the viruse...

journal_title：Journal of virology

authors： Benveniste RE,Callahan R,Sherr CJ,Chapman V,Todaro GJ

更新日期：1977-03-01 00:00:00

abstract：:The role of the cell nucleus in the development of African swine fever virus in Vero cells has been studied. No viral growth could be detected in enucleated cells under conditions that allow normal development of Sindbis virus. Furthermore, African swine fever virus DNA synthesis was inhibited more than 95% after infe...

journal_title：Journal of virology

authors： Ortin J,Vińuela E

更新日期：1977-03-01 00:00:00

abstract：:The synthesis of Sendai virus RNA species was examined after UV irradiation of cells late in infection. Compared with the inactivation of 50S genomic RNA synthesis, the synthesis of the group of mRNA species that sediments at 18S was inactivated at an average rate consistent with a process of sequential transcription ...

journal_title：Journal of virology

authors： Glazier K,Raghow R,Kingsbury DW

更新日期：1977-03-01 00:00:00

abstract：:Cell fractionation and protein electrophoresis were used to study the intracellular sites of synthesis and intermediate structures in the assembly of the virion proteins of vesicular stomatitis virus. Each of the three major virion proteins assembled into virions through a separable pathway. The nucleocapsid (N) prote...

journal_title：Journal of virology

authors： Knipe DM,Baltimore D,Lodish HF

更新日期：1977-03-01 00:00:00

abstract：:The effect of rifampin on the replication of vaccinia DNA was studied in mouse L cells by a cytochemical techinque and by alkaline sucrose sedimentation analysis of newly synthesized viral DNA molecules. By the use of a fluorescent DNA-binding compound (Hoechst 33258), the sequential appearance, size, and location of ...

journal_title：Journal of virology

authors： Esteban M

更新日期：1977-02-01 00:00:00

abstract：:A method for obtaining large quantities of defective interfering (DI) rabies virus particles that fulfill all the criteria delineated by Huang and Baltimore (1970) is described. The purified rabies DI virion was found to be much shorter (60 to 80 nm) than the complete virion (180 nm) and to have a viral genome of abou...

journal_title：Journal of virology

authors： Wiktor TJ,Dietzschold B,Leamnson RN,Koprowski H

更新日期：1977-02-01 00:00:00

abstract：:The morphogenesis of vesicular stomatitis virus was examined using freeze-fracture techniques, and the results obtained were compared with those from previously published experiments carried out with influenza viruses and togaviruses. The process of conversion of the host cell plasma membrane into the vesicular stomat...

journal_title：Journal of virology

authors： Brown DT,Riedel B

更新日期：1977-02-01 00:00:00

abstract：:A soluble polymerase-template complex prepared from poliovirus-infected cells was found to incorporate radioactive UTP into trichloroacetic acid-insoluble RNA linearly for 8 h in the presence of ATP and Mg2+. Radioactive CTP or GTP was not incorporated under identical conditions. Nearest-neighbor analysis of the in vi...

journal_title：Journal of virology

authors： Yin FH

更新日期：1977-01-01 00:00:00

abstract：:Using a method for freeze-drying intact cells, uninfected and murine leukemia virus (MuLV)-infected JLSV9 cell surfaces, as well as murine mammary tumor virus (MuMTV)-infected cell surfaces, were examined by electron microscopy. The 10-nm knobs of MuLV and the 5-nm spikes of MuMTV were clearly revealed on the surfaces...

journal_title：Journal of virology

authors： Demsey A,Kawka D,Stackpole CW

更新日期：1977-01-01 00:00:00

abstract：:Previously it was shown that the host-range gene of the Bratislava strain of avain sarcoma virus (B77 virus) spontaneously mutates with a very high rate. The wild-type B77 virus called B77 virus-II, mutates either to virus that efficiently infects duck cells (B77 virus-III) or to virus that does not mutate to the abil...

journal_title：Journal of virology

authors： Zarling DA,Mosser AG,Temin HM

更新日期：1977-01-01 00:00:00

abstract：:A study of virus-specific protein synthesis in infectious pancreatic necrosis virus-infected RTG-2 cells was undertaken to find a relationship between the coding capacity of virus genome (two segments of double-stranded RNA of 2.5 X10(6) and 2.3 X 10(6) molecular weight) and the sizes and relative amounts of polypepti...

journal_title：Journal of virology

authors： Dobos P

更新日期：1977-01-01 00:00:00

abstract：:A streptomycin-resistant Escherichia coli mutant has been isolated that is temperature sensitive for Qbeta phage, but not for the group I RNA phages f2, MS2, and R17. The growth of Qbeta in the mutant at the nonpermissive temperature (42 degrees C) results in the release of a near-normal burst of noninfectious particl...

journal_title：Journal of virology

authors： Engelberg-Kulka H,Dekel L,Israeli-Reches M

更新日期：1977-01-01 00:00:00

abstract：:Densonucleosis viruses 1 and 2 both contain the three polyamines putrescine, spermidine, and spermine in complete virus particles but not in their respective top components. The polyamines, with spermidine predominant, comprise 1.41% of the virus particle by weight, which is sufficient to neutralize 26% of the single-...

journal_title：Journal of virology

authors： Kelly DC,Elliott RM

更新日期：1977-01-01 00:00:00

abstract：:The nature and theory of flow field-flow fractionation is described, and its potential applicability to virus-like particles is discussed. Different virus types are shown to be retained at different levels. Retention can be controlled by variation of the experimental parameters, in good agreement with theory. However,...

journal_title：Journal of virology

authors： Giddings JC,Yang FJ,Myers MN

更新日期：1977-01-01 00:00:00

abstract：:A closed circular, double-stranded infectious DNA of Moloney leukemia virus has been described previously. The present report characterizes a second type of infectious, unintegrated viral DNA which is linear, largely double stranded, and of mass comparable to that of the closed circular viral DNA. The linear form is o...

journal_title：Journal of virology

authors： Smotkin D,Yoshimura FK,Weinberg RA

更新日期：1976-12-01 00:00:00

abstract：:Glycosylation of the envelope glycoprotein of vesicular stomatitis virus was examined using virus-infected HeLa cells that were pulse-labeled with radioactive sugar precursors. The intracellular sites of glycosylation and the stepwise elongation of the carbohydrate side chains of the G protein were monitored by membra...

journal_title：Journal of virology

authors： Hunt LA,Summers DF

更新日期：1976-12-01 00:00:00

abstract：:A new temperate bacteriophage, rho11, isolated by J. Hoch, has been characterized. This new phage is very similar to the temperate phage phi3T in size (380 nm), host range, homoimmunity, DNA buoyant density (1.694 g/ml), antigenicity, and molecular weight (around 6.0 X 10(7)) as determined in gels. Like phi3T, rho11 c...

journal_title：Journal of virology

authors： Dean DH,Orrego JC,Hutchison KW,Halvorson HO

更新日期：1976-11-01 00:00:00

abstract：:RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral heavy (l) and light (r) 32P-labeled DNA strands. Hybridization kinetics were analyzed by computer to estimate the number of viral RNA abundance classes, their relative concentrations, and the fraction of each DNA strand fro...

journal_title：Journal of virology

authors： Wold WS,Green M,Brackmann KH,Cartas MA,Devine C

更新日期：1976-11-01 00:00:00

abstract：:Two virus-specific species of newly synthesized DNA were isolated from rat fibroblast cell cultures infected with the Kilham rat virus (RV). These two DNA species were purified; their behavior on hydroxyapatite chromatography and their sedimentation coefficients in sucrose gradients were determined. One of the two spe...

journal_title：Journal of virology

authors： Gunther M,May P

更新日期：1976-10-01 00:00:00

abstract：:The ability of certain vesicular stomatitis virus (VSV; Indiana serotype) temperature-sensitive (ts) mutants to synthesize intracellular viral complementary RNA (vcRNA) at permissive or nonpermissive temperatures for productive infections has been investigated. Mutants belonging to complementation groups II, III, and ...

journal_title：Journal of virology

authors： Repik P,Flamand A,Bishop DH

更新日期：1976-10-01 00:00:00

abstract：:Semlike forest virus capsid protein cosedimented with the large ribosomal subunit at 60S in sucrose gradients after treatment of cytoplasm from infected cells with Triton X-100 and EDTA. In CsCl gradients the capsid protein banded with the subunit at a density of 1.56 to 1.57 g/cm3. Most of the capsid protein could be...

journal_title：Journal of virology

authors： Ulmanen I,Söderlund H,Kääriäinen L

更新日期：1976-10-01 00:00:00